Translational_Unit

Part:BBa_K302017:Design

Designed by: Steven Woodhouse, Alan Koh, Rachel May Boyd, Philip Hall   Group: iGEM10_Newcastle   (2010-08-18)


spaIFEG cluster


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 740
    Illegal BglII site found at 1488
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1755
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1035
    Illegal SapI site found at 1315
    Illegal SapI.rc site found at 2038


Design Notes

SpaI uses spoVG RBS (BBa_K143021), spaFEG uses native RBS (spaF and spaE RBS are in the coding sequence of preceding protein coding sequence). SpaG has had its TAG stop codon replaced with TAATAA.


Source

Gene bank

References